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Protein expression in yeast
PROTEOGENIX and protein production in yeast
The protein production platform in yeast represents an attractive solution for protein expression in eukaryotic cell. Indeed, yeast can produce significant quantities of recombinant protein at a reasonable cost compared to Baculovirus system (insect cells) or mammalian cells (HEK293, CHO).
Pichia pastoris, Saccharomyces cerevisiae, K. lactis and Pichia methanolica yeast strains
Yeast is a unicellular organism (fungus) capable of performing fermentation of animal or plant organics. Saccharomyces cerevisiae, known as “baker yeast” since it is used to make bread, is a widely used yeast strain to produce custom recombinant proteins.
Advantages and drawbacks of yeast expression systems
Unlike E. Coli, yeast can produce recombinant proteins with post-translational modifications (PTM: simple glycosylations, carboxylation, acylations…), even though these modifications are not as well reproduced as with advanced eukaryotic systems (baculovirus, mammalian cells). Yields are high with this system making it an attractive alternative compared to other eukaryotic solutions. One major drawback was that yeast was capable of performing N-glycolsylations only partially, in the sense that it produced “basic” forms containing many mannoses that did not correspond to glycosylations found on mammalian proteins. However, new systems recently developed can now reproduce post-translational modifications similar to those produced in mammalian cells. Interest in yeast production systems is again up-to-date for the production of protein for therapeutic purposes since it preserves the advantages of better yields and above all faster productions compared mammalian cells or baculovirus / insect cells expression systems.
More information about the protocol of protein expression in Yeast here.