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Custom monoclonal antibody 30 cell lines

Proteogenix, Newsletter

Custom monoclonal antibody
30 cell lines GUARANTEED in ELISA

Guaranteed service of monoclonal antibody in ELISA, 30 clones

Custom Monoclonal antibody Package content:

  • Antigen production in E. Coli:

    We design particular antigens to have the highest propability to get custom monoclonal antibodies that will detect accessible epitopes. We usually build a PEC –Poly Epitope Complex- a special assembly of up to 14 selected peptides to be produced in E. Coli. All peptides are separated by specific spacers and cloned in a proprietary vector containing a particular proprietary carrier: HIRE technology (High Immunognenicity Response Enabler). Both systems combined allow to dramatically increase immunogenicity, to ensure the best specificity and select the most accessible epitopes to have high affinity custom monoclonal antibodies.

    ELISA screening and monoclonal antibody development

  • Immunizations
  • Fusion
  • ELISA Screening
  • Subcloning of every positive clone :

    The main advantage of this is to be able to select the best clones based on final products and not on parental clones that could lead to completely different subclones, maybe not even secreting any antibody…

  • Mammalian cell line transfection :

    Cloning of the cDNA into an eucaryotic vector and transfection of a cell line to over express the protein.

  • ELISA and dot-blot Screening :

    Selection of all positive clones working in ELISA and dot-blot against the immunogen.

  • Ascites :

    We will then send you 50µl of unpurified ascites of every positive clone (at least 10) in order for you to choose the best one(s) to work in your application. If you want more than 3 clones delivered in this package, we will deliver each additional clone at extra cost.

Our Guarantees:

  • LEVEL 1:

    We guarantee to deliver unpurified ascites from at least 30 different clones which will work against the immunogen in ELISA. In case we fail, we will refund everything except 300€.

  • LEVEL 2:

    If we meet the level 1 guarantee but you don’t find at least one clone that works in your lab in your application against the endogenous protein, we will refund you 50% of the total cost.

Deliverables:

  • 2 frozen vials of 8 custom monoclonal antibody hybridoma cell lines
  • 2mg of purified ascites produced from from 3 of these 8 clones

Timeline:

120 days for the total package, antigen production and purified antibody production included.

Data provided:

For each cell line:

  • Epitope and affinity data (if available)
  • ELISA and dot blot data on immunogen

For more information on custom monoclonal antibody guaranteed, you can :

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