ANTIBODY CONJUGATION AND FRAGMENTATION
Proteogenix offers antibody conjugation services to different kind of molecules and enzymes :
- Horseradish Peroxydase (HRP)
- Phosphatase Alcaline
- Biotin
- KLH
- BSA
We also conjugate your antibodies to fluorochromes / fluorescent dyes.
FITC
EXCITATION (LASER) : 488 nm(Blue Diode / HeAr laser)
EMISSION : 525 nm
NOTE : Fluorescein isothioyanate; the most commonly used fluorochrome for FACS analysis detected in FL1 channel
Dyomics 547
EXCITATION (LASER) : 557 nm (Yellow laser)
EMISSION : 574 nm
NOTE : Alternative to Cy3 and Alexa Fluor® 535
PE
EXCITATION (LASER) : 488 nm (Blue Diode / HeAr laser)
EMISSION : 575 nm
NOTE : R-Phycoerythrin; PE is a large protein (240 kDa) containing 25 fluorophores; PE is the most commonly used dye for FACS analysis detected in FL2 channel on most instruments;suitable for detection of low density antigens
PE-Dyomics 590
EXCITATION (LASER) : 488 nm (Blue Diode / HeAr laser)
EMISSION : 599 nm
NOTE : R-Phycoerythrin - Dyomics 590; tandem dye of PE and Dyomics 590, same spectral properties as PE-Texas Red (ECD) tandem dye; detected in FL3 channel on single laser instruments
PE-Dyomics 647
EXCITATION (LASER) : 488 nm (Blue Diode / HeAr laser)
EMISSION : 672 nm
NOTE : R-Phycoerythrin - Dyomics 647; tandem dye of PE and Dyomics 647 detected in FL3 or FL4 on most instrumentssimilar to PE-Cy5 (PC5) and PE-Alexa Fluor® 647
APC
EXCITATION (LASER) : 633 nm (Red laser Red Diode / HeNe laser)
EMISSION : 660 nm
NOTE : Allophycocyanin; APC is a large protein (105 kDa) containing many fluorophores; APC is emerging as one of the brightest fluorescent probes for FACS analysis; detected in FL4 on dual laser instruments
Dyomics 647
EXCITATION (LASER) : 633 nm (Red laser, Red Diode / HeNe laser)
EMISSION : 672 nm
NOTE : Dyomics 647; DY647 is pentamethine based small fluorochrome detected in FL4 on dual laser instruments;except for FACS analysis, where it may replace use of traditional APC (namely good choice for intracellular staining), it is excellent for microscopy and microarrays;similar to Cy5 and Alexa Fluor® 647
If you do not find the molecule you want to conjugate your antibody or protein with, please contact us to check if we are able to provide it to you and do the conjugation for you.
We offer an antibody fragmentation service by digestion / treatment with :
- pepsin to obtain Fab’2 fragments
or
- papaïn to obtain Fab fragments